Composite

Part:BBa_K4297056:Design

Designed by: Yang Gu   Group: iGEM22_NJXDF-CHN   (2022-10-10)


PYLXP'-ARO10-ARO8-PAR4


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 1293
    Illegal NotI site found at 1448
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 5677
    Illegal BglII site found at 5884
    Illegal BamHI site found at 249
    Illegal BamHI site found at 7079
    Illegal BamHI site found at 7864
    Illegal BamHI site found at 7935
    Illegal BamHI site found at 8305
    Illegal BamHI site found at 8326
    Illegal XhoI site found at 4316
    Illegal XhoI site found at 4349
    Illegal XhoI site found at 6775
    Illegal XhoI site found at 9032
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 4945
    Illegal AgeI site found at 950
    Illegal AgeI site found at 4585
    Illegal AgeI site found at 5535
    Illegal AgeI site found at 6410
    Illegal AgeI site found at 8031
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 1
    Illegal BsaI site found at 1284
    Illegal BsaI site found at 4588
    Illegal BsaI site found at 6413
    Illegal BsaI site found at 6467
    Illegal BsaI site found at 7069
    Illegal BsaI site found at 8034
    Illegal BsaI.rc site found at 255
    Illegal BsaI.rc site found at 4582
    Illegal BsaI.rc site found at 5082
    Illegal BsaI.rc site found at 5817
    Illegal BsaI.rc site found at 6407
    Illegal BsaI.rc site found at 6953
    Illegal BsaI.rc site found at 8028
    Illegal SapI.rc site found at 7885


Design Notes

none


Source

none

References